background

 

Publications

General publications
WP 1: Generation of a high quality in vivo (animal and human) database
WP 2: Generation of a high quality in vitro database
WP 3: Iteritive amendment of the testing strategy
WP 4: New cell systems and new endpoints
WP 5: Role of adsorption, distribution and elimination
WP 6: Role of metabolism
WP 7.1: Role target organ toxicity (brain)
WP 7.2: Role target organ toxicity (kidney)
WP 7.3: Role target organ toxicity (liver)
WP 9: Pre-validation of test strategy

 

General publications

Articles

Clemedson C., Blaauboer B., Castell J.V., Prieto P., Risteli L., Vericat J.A. and Wendel A. (2006) ACuteTox- Optimisation and pre-validation of an in-vitro test strategy for predicting human acute toxicity. 5th World Congress on Alternatives & Animal Use in the Life Sciences, Berlin, August 21-25, 2005. ALTEX 23, Special Issue 2006.

Clemedson C., Kolman A. and Forsby A. (2007) The Integrated Acute Systemic Toxicity project (ACuteTox) for the optimisation and validation of alternative in vitro tests. ATLA 35, 33-38.

Kinsner-Ovaskainen A., Bulgheroni A., Hartung T., Prieto P. (2009) ECVAM's ongoing activities in the area of acute oral toxicity. Toxicol In Vitro. 23(8):1535-40

Schildknecht S., Poeltl D., Nagel D.M., Matt F., Scholz D., Lotharius J., Salvo-Vargas A., Leist M. (2009). Requirement of a dopaminergic neuronal phenotype for toxicity of low concentrations of 1-methyl-4-phenylpyridinium to human cells. Toxicol Appl Pharmacol, 241, 23-35

Henn A., Lund S., Hedtjärn M., Schrattenholz A., Pörzgen P., and Leist M. (2009). The suitability of BV2 cells as alternative model system for primary microglia cultures or animal experiments of brain inflammation – ALTEX, 26, 83-94

Volbracht C., Penzkofer S., Mansson D., Christensen K.V., Fog K., Schildknecht S., Leist M., Nielsen J. (2009). Measurement of cellular beta-site of APP cleaving enzyme 1 activity and its modulation in neuronal assay systems. Anal Biochem, 387, 220-234.

Hartung T., Blaauboer B., Leist M. (2009). Food for thought ... on education of alternatives in toxicology. – ALTEX 26, 255-263.


Conference contributions

Clemedson C., A-Cute-Tox- Optimisation and pre-validation of an in vitro test strategy for predicting human acute toxicity, INVITOX 2004 Zegrze in Poland, In Vitro (ESTIV), 8-11 September 2004.

Clemedson C. A-Cute-Tox - Optimisation and pre-validation of an in vitro test strategy for predicting human acute toxicity. 10th International Congress of Toxicology, Tampere, Finland.11-15 July 2004. Poster.

Clemedson C., Blaauboer B., Castell J., Prieto P., Risteli L., Vericat J-A and Wendel A. ACuteTox- Optimisation and pre-validation of an in vitro test strategy for predicting human acute toxicity, , The 5th World Congress on Alternatives & Animal Use in the Life Sciences, Berlin, August 21-25, 2005. Oral presentation

Clemedson C., Blaauboer B., Castell J., Prieto P., Risteli L., Vericat J-A and Wendel A. ACuteTox- An EU 6FP project on optimisation and pre-validation of in vitro test strategy for predicting human acute toxicity, The SSCT/ETS workshop in Toila, Estonia, October 20-23, 2005. Oral presentation.

Clemedson C., Short summary report on the progress within the ACuteTox project 2005, The Ecopa meeting, Brussels airport, Belgium, 16-18 December, 2005. Oral presentation.

Forsby A. The ACuteTox project. Toxikologiska Rådet, National Chemicals Inspectorate in Stockholm, Sweden, 21 April 2005. Oral presentation.

Clemedson, C., Cole, T., Clothier, R., Coecke, S., O´Connor, J.E., Blaauboer, B., Gómez-Lechón, M-J., Vericat, J.A., Forsby, A., Ryan, M P, Castell, J., Prieto, P., Risteli, L. and Wendel, A. Optimisation and pre-validation of an in vitro test strategy for predicting human acute toxicity: progress report from the ACuteTox project. Abstract at the 14th International Workshop on In Vitro Toxicology, Ostende, Belgium, 2-5 October, 2006. Oral presentation.

Clemedson, C., Cole, T., Clothier, R., Coecke, S., O’Connor, J.E., Blaauboer, B., Gómez-Lechón, M.J., Vericat, J.A., Forsby, A., Ryan, M.P., Castell, J., Prieto, P., Risteli, L., Wendel. A. (2006) Optimisation and pre-validation of an in vitro test strategy for predicting human acute toxicity: Progress report from the ACuteTox project. 46th ETCS International Meeting/3rd International Joint Meeting AICC-Celltox on "In Vitro Cytotoxicity Mechanisms" Verona, March 2006.

Kinsner A., Clemedson, C., Cole, T., Clothier, R., Coecke, S., O’Conner, J., Blaauboer, B., Gómez-Lechón, M., Vericat, J., Forsby, A., Ryan, M., Castell Ripoll, J., Prieto Peraita, M., Risteli, L. Optimisation and Pre-validation of an In Vitro Test Strategy for Predicting Human Acute Toxicity: Progress of the A-Cute-Tox Project. 44th Congress of the European Societies of Toxicology "EUROTOX 2007", Amsterdam, the Netherlands, 7-10 October 2007. Poster.

Clemedson C., Blaauboer B., Castell J.V., Casati S., Cole T., Clothier R., Coecke S., O´Connor J.E., Forsby A., Gómez-Lechón M.J., Hartung T., Kinsner A., Prieto P., Ryan M.P., Risteli L., Vericat J.A and Wendel A. Towards an Alternative Approach for Oral Acute Systemic Toxicity: The A-Cute-Tox Project. 6th World Congress on Alternatives & Animal Use in the Life Sciences, Tokyo, Japan, 20th August 2007. Oral presentation by Sandra Coecke.

Cecilia Clemedson, Thomas Cole, Richard Clothier, Sandra Coecke, José Enrique O´Connor, Bas Blaauboer, Maria José Gómez-Lechón, Joan Albert Vericat, Anna Forsby , Michael P. Ryan, José Castell, Pilar Prieto, Leila Risteli and Albrecht Wendel. The ACuteTox project-Development and pre-validation of an in vitro testing strategy for oral acute systemic toxicity. 25th Workshop of the Scandinavian Society for Cell Toxicology Salzau, September 2007.

Kinsner, Anna Bulgheroni, P. Prieto. ECVAM ongoing activities to meet the cosmetics 2009 deadline related to acute oral toxicity. ESTIV2008 congress, Stockholm, September, Oral presentation.

Kinsner-Ovaskainen A. and Prieto P. ECVAM’s current activities in the area of systemic toxicity. IVTIP (UK) in May 2008. Oral presentation.

Prieto P. ECVAM Acute Toxicity Projects, EPAA meeting (Belgium) May 2008. Oral presentation.

Prieto P. and Kinsner-Ovaskainen A. Presentation of an update of the ACuteTox project. COLIPA PT Systemic Toxicity meeting (Brussels) May 2008.

Clemedson C., Cole T., Clothier R., Coecke S, O´Connor J.E., Blaauboer B., Gómez-Lechón M.J., Vericat J.A., Forsby A., Ryan M.P., Castell J., Kinsner A., Prieto P. 2009. Overview of the ACuteTox project and multivariate analysis of the in vitro data. WC7 in Rome 29 August-3 September. Oral presentation.

Others

ACuteTox project an Integrated Project under the EU 6FP with the aim to optimise and pre-validate an in vitro test strategy for predicting human acute toxicity, Cecilia Clemedson, European Society of Toxicology in vitro, Newsletter no 18, p 6, November 2005.

ACuteTox Project Progress, Cecilia Clemedson, European Society of Toxicology in vitro, Newsletter no 19, p 8, March 2006.

Index

 

WP 1: Generation of a high quality in vivo (animal and human) database

Submitted manuscript

Hoffmann S., Kinsner-Ovaskainen A., Prieto P., Mangelsdorf I., Bieler C., Cole T. (2010) Acute oral toxicity: variability, reliability, relevance and interspecies comparison of rodent LD50 data from literature surveyed for the ACuteTox project. Regulatory Toxicology and Pharmacology (submitted).

Conference contributions

Kolman A., Use of human acute toxicity data for the evaluation of in vitro assays in the ACuteTox project, Joint conference of the Estonian Society of Toxicology & Scandinavian Society for Cell Toxicology, Toila, Estonia, October 20-23, 2005.

Kolman A. Implementation of in vitro tests for human acute toxicity assessment – MEIC and ACuteTox projects. 10th Interdisciplinary Czech-Slovak Toxicological Conference, Palacky University, Olomouc, September 14-16, 2005. Biomedical Papers, v. 149, suppl. 1, p. 20, 2005.

Kolman A., Sjöström M., Cole T., Hoffmann S., Rudowski R., Rzepka R., Kinsner A., Bieler C., Mangelsdorf I., Clemedson C., Prieto P. and Coecke S. Use of human data collected in AcuBase (ACuteTox Project) for analyses of animal and in vitro data. 25th SSCT Workshop on in vitro toxicology, 19-22 September Salzau, Germany 2007.

Index


 
WP 2: Generation of a high quality in vitro database

Articles

Clothier R., Dierickx P., Lakhanisky T., Fabre M., Betanzos M., Curren R., Sjostrom M., Rabbe H. Bourne N., Hernandez V., Mainez J., Owen M., Watts S., Anthonissen R., Database of IC50 values and principle component analysis of six basal cytotoxicity assays, for use in modelling of in vitro-in vivo data of the ACuteTox project. ATLA 36:503-519 (2008).

Index

 

WP 3: Iteritive amendment of the testing strategy

Articles

Rzepka R., Rudowski R., Kinsner A., Sladowski D., Kolman A., Cole T., Bieler C., Prieto P., Coecke S. AcuBase – on-line system for high quality acute toxicity data acquisition and management. Med-e-Tel, The International, Educational and Networking Forum for eHealth, Telemedicine and Health ICT Proceedings, M. Jordanova M, F. Lievens (Eds.), Luxexpo 2007, pp.202-204.

Sjöström, M., Clemedson, C, Clothier, R and Kolman A. Toxicology in Vitro , 22 (2008) 1405-1411. Estimation of human blood LC50 values for use in modelling of in vitro-in vivo data of the ACuteTox project.

Kinsner-Ovaskainen A., Rzepka R., Rudowski R., Coecke S., Cole T., Prieto P. (2009) AcutoxBase, an innovative database for in vitro acute toxicity studies. Toxicol In Vitro. 23(3):476-85.

Conference contributions

Rzepka R., Rudowski R., Sladowski D. and Coecke S. AcuBase - database and online service of in vitro tests and methods for predicting human acute toxicity. 5th World Congress on Alternatives & Animal Use in the Life Sciences, Berlin, August 21-25, 2005. ALTEX 22, Special Issue 2005. Oral presentation.

Kinsner A., Rudowski R., Rzepka R., Kolman A., Clemedson C., Cole T., Mangelsdorf I., Bieler C., Śladowski D., Prieto P., Coecke S. AcuBase - a unique database for integration and analysis of acute toxicity data. Abstract at the 14th International Workshop on In Vitro Toxicology, Ostende, Belgium, 2-5 October, 2006.

Kolman, A., Rudowski, R., Rzepka, R., Kinsner, A., Cole, T., Clemedson, C., Mangelsdorf, I., Bieler, C., Prieto, P., and Coecke, S. AcuBase- A noval on-line database within ACuteTox Project. Abstract at the SSCT workshop, Grinda, Sweden, 7-9 September, 2006.

Rudowski R. Presentation of ―AcuBase – on-line system for high quality acute toxicity data acquisition and management‖ R. Rzepka, R. Rudowski, A. Kinsner, D. Sladowski, A. Kolman, T. Cole, C. Bieler, I. Mangelsdorf, P. Prieto and S. Coecke, at the Health Conference Medetel 2007 in Luxemburg. The presentation has been published in the Proceedings of the Conference.

Kolman A. Presentation of a poster ―Database (AcuBase) of documented in vitro and in vivo acute toxicity compiled for ACuteTox project reference chemicals‖ at the 25th SSCT meeting in Salsau, Germany, 19-22 September 2007.

Sjöström M., Kolman A., Clemedson C. Multivariate methods for analyses of in vitro data and for in vitro-in vivo modeling. 15th International Congress on In Vitro Toxicology, 25 - 28 Sept 2008, Stockholm, Sweden. Oral presentation.

Seminars & Lectures

Rzepka R., Rudowski R., Kinsner A., Sladowski D., Kolman A., Cole T., Bieler C., Mangelsdorf I., Prieto P., Coecke S. AcuBase-on line system for high quality acute toxicity data acquisition and management. The International Educational and Networking Forum for eHealth, Telemedicine and Health ICT. Proceedings of Medetel 2007, 18-20 April 2007, Luxemburg, pp. 202-204.

Index

 

WP 4: New cell systems and new endpoints

Articles

Devos S., Cormont F., Vrtala S., Hooghe-Peters E., Pirson F. and Van Snick J. (2006) Allergen-induced IL-9 production in vitro: Correlation with atopy in human adults and comparison with IL-5 and IL-13. Clinical and Experimental Allergy 36, pp 1-9.

Herrera G., Diaz L., Martinez-Romero A., Gomes A., Villamon E., Callaghan R.C. and O’Connor, J.E. (2007). Cytomics: A Multiparametric, Dynamic Approach to Cell Research. Toxicol. 21:176-182.

Gómez-Lechón M.J., Donato M.T., Martínez-Romero A., Jiménez N., Castell J.V., O’Connor J.E (2007). A human hepatocellular in vitro model to investigate steatosis. Chem.-Biol. Interact. 165: 106–116.

Herrera G., Pinto S., Diaz L., Gomes A., Parra M., Arenas M., Callaghan R.C., O´Connor J.E. (2007). Assessment of Oxidative Damage to DNA by Flow Cytometry and High Content Bioimaging Assays. Free Rad. Res., 41: 23.

Pinto S., Diaz L., Herrera G., Gómez-Lechón M.J., Donato T., Castell J.V., O´Connor J.E. (2007). High Content Bioimaging Assays for Oxidative Stress Assessment in Primary Cultures of Hepatocytes. Free Radical Research, 41: 23.

Diaz L., Gomes A., Pinto S., Parra M., Callaghan R. C., Herrera G. and O´Connor J.E. (2007). In Vitro Strategies for Predicting Human Acute Toxicity: Screening Oxidative Cytotoxity by Flow Cytometry and High Content Assays. Free Radical Research, 41: 23.

Cerrato L., Valeri A., Bueren J., Albella B. (2009). In vitro sensitivity of granulo-monocytic progenitors as a new toxicological cell system and endpoint in the ACuteTox project. Toxicology and Applied Pharmacology, 238:111-119.

Conference contributions

Guerra A., Campillo N.E., Páez J.A., Artificial neural networks in medicinal chemistry, XVI Congreso Nacional de la Sociedad Española de Química Terapéutica held in Bilbao (Spain), September 2005.

Martínez-Romero A., O’Connor J.E., Herrera G., Díaz, L., Alvarez-Barrientos A., Callaghan R.C. ―The role of Cytomics in the test strategies for chemical risk evaluation: The European integrated project A-Cute-Tox‖. IX Congresso da Sociedade Ibérica de Citometría, Porto (Portugal), May 2005.

Herrera G., Martínez A., Bodi C., Díaz-Vico L., Gil-Casanova D., Callaghan R.C., O’Connor J.E. ―Novel flow cytometric assays of oxidative stress and oxidative damage to DNA for assessing sustained in vitro cytotoxicity‖. XXVIII Congreso de la Sociedad Española de Bioquímica y Biología Molecular, Zaragoza, Spain, September 2005.

O’Connor J.E., Herrera G., Martínez-Romero A., Díaz L., Alvarez-Barrientos A., Callaghan R.C. ―La Citómica en las estrategias generales de detección de toxicidad aguda: El proyecto integrado A-Cute-Tox‖. IX Congresso da Sociedade Ibérica de Citometría, Porto (Portugal), May 2005

O’Connor J.E. ―Citómica: Una nueva frontera en Biología Celular‖. Workshop ―Nuevas Tecnologías‖, XI Congreso de la Sociedad Española de Biología Celular, Cádiz, (Spain), November 2005.

Cerrato L., Gómez S.G., Bueren J.A. and Albella B. Role of the in vitro hematopoietic cultures in the european project ACuteTox. 3rd Congress of the European Societies of Toxicology and 6th Congress of Toxicology in Developing Countries, Cavtat, Croatia, September 2006. (Toxicol. Lett. 164S: S2044)

Cerrato L., Gómez S.G., Bueren J.A. and Albella B. Papel de los cultivos hematopoyeticos en la prevalidación de Estrategias in vitro para predecir toxicidad aguda en humanos. XIX Congreso Nacional. Asociación Española de Técnicos de Laboratorio. May 2006.

Cerrato L., Gómez S.G., Bueren J.A. and Albella B. In vitro myeloid haematopoietic cultures (CFU-GM) in the european Project A-Cute-Tox. 3rd Meeting of the ACuteTox Consortium, Ostende, October, 2006.

Herrera G., Martinez-Romero A., Diaz L., Villamon E., Callaghan R.C., O’Connor J.E. Cytomics: A Multiparametric, Dynamic Approach to Cell Research. XXIX Congreso de la Sociedad Española de Bioquímica y Biología Molecular, Alicante, September 2006.

Herrera G., Martinez-Romero A., Callaghan R.C., Villamon E., Diaz L., Valero L., Gil D., Navarro C., Bodi C., O’Connor J.E. Cytomics: A Multiparametric, Dynamic Approach to Cell Research. 46th ETCS International Meeting/3rd International Joint Meeting AICC-Celltox on "In Vitro Cytotoxicity Mechanisms" Verona, March 2006.

Herrera G., Díaz L. , Gil D., Navarro C., O’Connor J.E. 96-well Plate Flow Cytometry: A Novel Cytomic Approach for Rapid Screening for In Vitro Toxicology. 46th ETCS International Meeting/3rd International Joint Meeting AICC-Celltox on "In Vitro Cytotoxicity Mechanisms" Verona, March 2006.

Martínez-Romero A., Álvarez-Barrientos A., Callaghan R. C., Prieto P., O'Connor J. E. Use of V79 Cells Expressing CYP2D6 for Cytomic Analysis of Biotransformation-Dependent and Independent Cytotoxicity. 46th ETCS International Meeting/3rd International Joint Meeting AICC-Celltox on "In Vitro Cytotoxicity Mechanisms" Verona, March 2006.

Devos S., Hooghe R., Hooghe-Peters E., Kooijman R., O’Connor J.E., Villamón E., Gómes A. Changes in cytokine production in vitro as indicators for immunotoxicity. III Meeting of the ACuteTox Consortium, Ostende, October 2006.

Herrera G., Díaz L., Gomes A., Villamón A., Callaghan R.C., O’Connor J.E. Miniaturized Screening of Cytotoxicity by Cytomic Assays. III Meeting of the A-Cute-Tox Consortium, Ostende, October 2006.

Herrera G., Pinto S., Díaz L., Gomes A., Callaghan R.C. and O’Connor J.E. Miniaturized citomic assays for determination of intracellular levels of the oxidized DNA base 8-oxoguanine as an indicator of oxidative stress. International Workshop on High Content Analysis. Madrid, Spain, March 2007.

Pinto S., Diaz L., Herrera G., Gomez-Lechón, M.J., Donato T., Castell J.V., Callaghan R.C. and O’Connor J.E. Application of high-content analysis to explore functional endpoints of cytotoxicity in primary cultures of hepatocytes. International Workshop on High Content Analysis. Madrid, Spain, March 2007.

Pinto S., Diaz L., Herrera G., Gómez-Lechón M.J., Donato T., Castell J.V. and O´Connor J.E. High Content Bioimaging Assays for Oxidative Stress Assessment in Primary Cultures of Hepatocytes. Free Radical Research, 41: 23. 2007.

Herrera G., Díaz L., Gomes A., Parra M., Pinto S., Callaghan R.C., O’Connor J.E. Miniaturized cytomic assays of oxidized DNA-base 8-oxoguanine by fluorescent bioimaging and flow cytometry. International Congress Focus on Microscopy-FOM 2007, Valencia, Spain, April 2007.

Herrera G., Pinto S., Díaz L., Gomes A., Parra M., Arenas M., Callaghan R. and O’Connor J.E. Miniaturized cytomic assays for determination of intracellular levels of the oxidized DNA base 8-oxoguanine as an indicator of oxidative stress. X Congreso de la Sociedad Ibérica de Citometría. Barcelona, June 2007.

Herrera G., Pinto S., Diaz L., Gomes A., Parra M., Arenas M., Callaghan R.C. and O´Connor J.E. Assessment of Oxidative Damage to DNA by Flow Cytometry and High Content Bioimaging Assays. Free Radical Research, 41: 23. 2007.

Alvarez S., Devos S., Gomes A., Kooijman R.,Villamón E., Hooghe R., Hooghe-Peters E. and O’Connor J.E. Multiplexed assays of cytokine secretion by human blood cultures for in vitro assessment of immunotoxicity. X Congreso de la Sociedad Ibérica de Citometría. Barcelona, June 2007.

Pinto S., Diaz L., Herrera G., Gómez-Lechón M.J., Donato T., Castell J.V. and O’Connor J.E. Application of High-Content Analysis to explore functional endpoints of cytotoxicity in primary cultures of hepatocytes. X Congreso de la Sociedad Ibérica de Citometría. Barcelona, June 2007.

Díaz L. The role of Cytomics to predict acute human toxicity: A cytomic assay panel for cytotoxicity screening. X Congreso de la Sociedad Ibérica de Citometría. Barcelona, June 2007.

Gomes A. In vitro test strategies for predicting human acute toxicity: Screening oxidative cytotoxicity with flow cytometric and High-Content assays. X Congreso de la Sociedad Ibérica de Citometría. Barcelona, June 2007.

Diaz L., Gomes A., Pinto S., Parra M., Callaghan R.C., Herrera G. and O´Connor J.E. In Vitro Strategies for Predicting Human Acute Toxicity: Screening Oxidative Cytotoxity by Flow Cytometry and High Content Assays. Free Radical Research, 41: 23. 2007.

Cerrato L., Bueren J.A. and Albella B. Toxicity of chemicals involved in the European project A-Cute-Tox on the myeloid hematopoietics. 44th Congress of the European Societies of Toxicology, Amsterdam, October 2007.

Cerrato L., Bueren J. A. and Albella B. PAPEL DE LOS CULTIVOS HEMATOPOYETICOS DE PRECURSORES MEGACARIOCÍTICOS EN LA PREVALIDACIÓN DE ESTRATEGIAS IN VITRO PARA PREDECIR TOXICIDAD AGUDA EN HUMANOS. XX Congreso Nacional, Asociación Espanola de Técnicos de la Laboratorio, 2007.

Herrera G., Díaz L., Gomes A., Pinto S., Parra M., Alvarez S., Algueró C., Callaghan R.C., O'Connor J., E. Integrated strategy of miniaturized cytomic in vitro assay and data mining for prediction of acute toxicity to humans: Application to risk assessment and toxic classification of chemicals. 13 Leipziger Workshop on Cytomics and NanoBioengineering, Leipzig, Germany 2008.

Cerrato L., Bueren J.A. and Albella B. Relationship between the in vitro effect of chemicals on human myeloid progenitors and the human peak plasma levels inducing toxicity. 44th Congress of the European Societies of Toxicology in Rhodes 2008 (EUROTOX2008).

HerreraG., Díaz L., Gomes A., Pinto S., Parra M., Callaghan R.C., O'Connor J,E. Integration of the IN Cell 1000 analyzer and flow cytometry for high-content analysis in toxicology in vitro. InCell Users Day 2008, Trinity College, Dublin.

Cerrato L., Pérez L., Bueren J.A., Albella B. Determinación de la sensibilidad in vitro de progenitores mieloides humanos en el contexto del proyecto europeo A-Cute-Tox. XXII Congreso Nacional de la Asociación Española de Técnicos de Laboratorio, Palma de Mallorca, Spain 2009.

Cerrato L., Valeri A., Bueren J.A., Albella B. In vitro sensitivity of granulo-monocytic progenitors as a new endpoint in the ACuteTox project. EUROTOX2009 Dresden (Alemania) 2009.

Book chapters

Alvarez-Barrientos, A., O’Connor, J.E. and Callaghan, R.C. (2005) ―Citómica: Técnicas y estrategias en citometría de sistemas‖. In: Las Omicas Genómica, Proteómica, Citómica y Metabolómica: Modernas Tecnologías Para el Desarrollo de Fármacos (M. Cascales, M.J. Gómez-Lechón y J.E. O'Connor, eds. Instituto de España-Real Academia Nacional de Farmacia, Madrid. pp. 123-154.

O’Connor, J.E., Alvarez-Barrientos, A., Callaghan, R.C., Herrera, G. and Martínez-Romero, A. (2005) ―La Citómica como estrategia alternativa en Farmacología y Toxicología in vitro‖. In: Las Omicas Genómica, Proteómica, Citómica y Metabolómica: Modernas Tecnologías Para el Desarrollo de Fármacos (M. Cascales, M.J. Gómez-Lechón y J.E. O'Connor, eds. Instituto de España-Real Academia Nacional de Farmacia, Madrid. pp.155-182.

O’Connor, J.E., Herrera, G., Martínez-Romero, A., Bodi, C., Valero, C., Callaghan, R.C. and Monteiro, M.C. (2005) ―La citometría de flujo en el analisis citómico: in vivo, in vitro, ¿in fluxo?‖ In: Las Omicas Genómica, Proteómica, Citómica y Metabolómica: Modernas Tecnologías Para el Desarrollo de Fármacos (M. Cascales, M.J. Gómez-Lechón y J.E. O'Connor, eds. Instituto de España-Real Academia Nacional de Farmacia, Madrid. pp. 183-206.

Lectures

O’Connor, J.E. ―Laboratorio de Citómica‖. Centro de Investigación ―Príncipe Felipe‖, Valencia (Spain), April 2005.

O’Connor, J.E. ―Citometria de Fluxo e Citocinas‖. Laboratório Doutor Chaves, Lisboa (Portugal), November 2005.

O’Connor, J.E "Análisis funcional por citometría de flujo". V Curso Teórico-Práctico de Citometría de Flujo. CIEMAT/CNIC, Madrid, November 2005.

References to the A-Cute-Tox Project have been introduced in invited lectures delivered in Ph.D. and Post-graduate Courses in Spain and Portugal:
Albella B. (2006) Papel de los cultivos hematopoyeticos en la predicción de la toxicidad en humanos: proyecto europeo A-Cute-Tox. Curso de Doctorado ―Proliferación Celular y Apoptosis‖, Univrsidad de Valencia, January 2006.

O’Connor J.E. (2006) Citometría Funcional. Curso de Aplicaciones Clínicas de la Citometria de Flujo, Hospital Vall d’Hebron, Barcelona, September 2006.

O’Connor, J.E. (2006) Citometría Funcional. Curso de Citometría de Flujo, CIEMAT-CNIC, Madrid, November 2006.

O’Connor J.E. (2006) Citometría Funcional. Mestrado em Genómica, Proteómica e Citómica no Laboratório, Cooperativa de Ensino Superior Politécnico e Universitário, Gandra-Paredes, September 2006.

References to the A-Cute-Tox Project have been introduced in invited lectures delivered in Ph.D. and Post-graduate Courses:
O’Connor J.E. ―Laboratorio de Citómica‖. Centro de Investigación ―Príncipe Felipe‖, Valencia (Spain), April 2005.

O’Connor J.E. ―Citometria de Fluxo e Citocinas‖. Laboratório Doutor Chaves, Lisboa (Portugal), November 2005.

O’Connor J.E "Análisis funcional por citometría de flujo". V Curso Teórico-Práctico de Citometría de Flujo. CIEMAT/CNIC, Madrid, November 2005.

Albella B. Papel de los cultivos hematopoyeticos en la predicción de la toxicidad en humanos: proyecto europeo A-Cute-Tox. Curso de Doctorado ―Proliferación Celular y Apoptosis‖, Universidad de Valencia, January 2006.

O’Connor J.E. Citometría Funcional. Curso de Aplicaciones Clínicas de la Citometria de Flujo, Hospital Vall d’Hebron, Barcelona, September 2006.

O’Connor J.E. ―Citometría Funcional‖. Curso de Citometría de Flujo, CIEMAT-CNIC, Madrid, November 2006.

O’Connor J.E. ―Citometria Funcional‖. Mestrado em Genómica, Proteómica e Citómica no Laboratório, Cooperativa de Ensino Superior Politécnico e Universitário, Gandra-Paredes, September 2006.

Albella B. Papel de los cultivos hematopoyeticos en la predicción de la toxicidad en humanos: proyecto europeo A-Cute-Tox. Curso de Doctorado ―Proliferación Celular y Apoptosis‖, Universidad de Valencia, March 2007.

O’Connor J.E. ―Citometría Funcional‖. Curso de Citometría de Flujo, CIEMAT-CNIC, Madrid, November 2007.

Others

Devos S. Expression of IL-9 and allergy - Effects of some organic pollutants on the immune response with special concern for allergy. Facultieit geneeskunde en famacie dienst farmacologie, Vrije Universiteit Brussel, 2006-2007. PhD Thesis.

Index

 

WP 5: Role of adsorption, distribution and elimination

Articles

Blaauboer B.J., Hermens J. and van Eijkeren J. (2006). Estimating acute toxicity based on in vitro cytotoxicity: role of biokinetic modelling. 5th World Congress on Alternatives & Animal Use in the Life Sciences, Berlin, August 21-25, 2005. ALTEX 23, Special issue 2006.

N.I. Kramer van Eijkeren J.C.H. and Hermens J.L.M. (2007). Influence of albumin on sorption kinetics in solid-phase microextraction: consequences for chemical analyses and uptake processes. Anal. Chem., 79 6941-6948.

Blaauboer, B.J. and Andersen, M.E. (2007).The need for a new toxicity testing and risk analysis paradigm to implement REACH or any other large scale testing initiative. Letter to the Editor, Archives of Toxicology 81, 385–387

Hubatsch I., Ragnarsson E., Artursson P. (2007). Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers. Nat Prot 2, 2111-2119.

Blaauboer B.J. (2008). The contribution of in vitro toxicity data in hazard and risk assessment: Current limitations and future perspectives. Toxicology Letters 180, 81-84.

Clewell H.J. III, Andersen M.E., Blaauboer B.J. (2008). On the Incorporation of Chemical-Specific Information in Risk Assessment Toxicology Letters, 180, 100-109.

Guerra A., Paez J.A. and Campillo N. E. (2008). Artificial Neural Networks in ADMET Modeling: Prediction of Blood-Brain Barrier Permeation QSAR & Combinatorial Science. QSAR Comb. Sci. 27(5):586-594.

Culot M., Lundquist S., Vanuxeem D., Nion S., Landry C., Delplace Y., Dehouck M-P., Berezowski V., Fenart L., and Cecchelli R. (2008). An in vitro blood-brain barrier model for high-throughput (HTS) toxicological screening. Toxicology in vitro 22, 799-811.

Schirmer K., Tanneberger K., Kramer N.I., Völker D., Scholz S., Hafner C., Lee L.E.J., Bols N.C., Hermens J.L.M., (2008). Developing a list of reference chemicals for testing alternatives to whole fish toxicity tests. Aquatic Toxicology 90, 128-137.

Campillo N.E., Páez J. A.. (2009). Frontiers in Drug Design & Discovery 4, 291-332 291.

Kramer N.I., Hermens J.L.M. and Schirmer K. (2009). The influence of modes of action and physicochemical properties of chemicals on the correlation between in vitro and acute fish toxicity data. Toxicology in Vitro, 23(7), 1372-1379.

Guerra A., Campillo N.E., Páez J.A. (2010). Neural Computational Prediction of Oral Drug Absorption based on CODES 2D Descriptors. Eur. J. Med. Chem, 45, 930-40.
Blaauboer, B.J. (2010) Biokinetic modelling and in vitro-in vivo extrapolations. Journal of Toxicology and Environmental Health Part B, 13: 2, 242 — 252

Tanneberger, K., Rico-Rico, A., Kramer, N.I., Busser, F.J.M., Hermens, J.L.M., Schirmer, K. Effects of solvents and dosing procedure on chemical toxicity in cell-based in vitro assays. (2010) Environmental Science and Technology, 44 (12), pp. 4775-4781.

Submitted manuscripts

Turco L, Catone T., Caloni F., Di Consiglio E.,Testai E. and Stammati A. Caco-2/TC7 cell line charaterization for intestinal absorption: How reliable is this in vitro model for the prediction for oral dose fraction absorbed in human? Submitted to Toxicology In Vitro.

Conference contributions

Blaauboer B. The role of biokinetic information in the interpretation of in vitro cytotoxicity data: an essential part of estimating acute toxicity in the ACuteTox programme. 5th World Congress on Alternatives & Animal Use in the Life Sciences, Berlin, August 21-25, 2005. ALTEX 22, Special Issue 2005. Oral presentation.

Campillo N.E., Guerra A. and Páez J.A. Applicación de diferentes modelos de redes neuronales artificiales (RNA) a problemas de la química médica. Conference at the faculty of Chemistry, University of Montevideo, Uruguay, 2005.

Campillo N.E. Codes: A Topological Method To Encode Chemical Structures Based on Neural Computing. Application in Biokinetic Parameters Prediction. Conference at IVTIP Plenary Meeting, Madrid, Spain, 2005.

Guerra A., Campillo N.E. and Páez J.A. Artificial neural networks in medicinal chemistry. XVI Congreso Nacional de la Sociedad Española de Química Terapéutica held in Bilbao (Spain), September 2005.

Caloni F., Catone T., De Angelis I., Di Consiglio E., Testai E., Turco L., Zucco F. and Stammati A. Relevance of absorption in acute toxicity studies. 46th ETCS International Meeting/3rd International Joint Meeting AICC-Celltox on "In Vitro Cytotoxicity Mechanisms" Verona, March 2006. Poster.

Kramer N.I., Krismartina M., Blaauboer B.J. and Hermens J.L.M. Measuring and modeling bioavailability in in vitro systems. Chem-Biol Interact 169, p.137. 2007. Abstract.

Invited speaker, BBB meeting, September 2008, Amsterdam (P35).

Invited speaker, Workshop - Bottlenecks in the application of 3Rs in the R&D process in the pharmaceutical, May 2008, Madrid (P35).

Invited speaker, 15th International's Molecular Medicine Trioconference, March 2008, San Francisco, USA (P35).

A poster communication has been presented at the meeting XXth International Symposium on Medicinal Chemistry held in Viena, September 2008. Artificial Neural Networks in ADMET Modeling: Prediction of Blood-Brain Barrier Permeation and Oral Absorption (P13).

Blaauboer B. J. Kinetic considerations for the interpretation of in vitro toxicity data in risk assessment. 15th International Congress on In Vitro Toxicology, 25 - 28 Sept 2008, Stockholm, Sweden. Oral presentation.

Turco L., Catone T., Caloni F., Testai E. and Stammati A. Caco-2/TC7 cell line characterization for intestinal absorption: a reliable in vitro model for the prediction of the oral dose fraction absorbed in human. 15th International Congress on In Vitro Toxicology, 25 - 28 Sept 2008, Stockholm, Sweden. Oral presentation.

Hallier-Vanuxeem D., Landry C., Culot M., Nion S., Dehouck M.P. and Cecchelli R. A rapid in vitro model for Blood-Brain Barrier screening of neurotoxic chemicals. 15th International Congress on In Vitro Toxicology, 25 - 28 Sept 2008, Stockholm, Sweden. Poster presentation.

Artursson P. Modelling of Absorption, Intracellular Accumulation and Excretion. 15th International Congress on In Vitro Toxicology, ESTIV 2008, Djurönäset, Sweden, September 25-28, 2008.

Doctoral Thesis

Ph.D. Angela Guerra Alvarez. 2009. Application of Artificial Intelligence in Medical Chemistry. Supervisors: J. A. Páez , N.E. Campillo, Universidad Autónoma de Madrid.

Others

Guerra A. Applicación de la inteligencia artificial a la predicción de propiedades ADMET: Paso de la barrera hematoencefálica. Universidad Autónoma de Madrid , Facultad de ciencias, Departemento de Química Orgánica, 2006. Diploma work (Text and Oral).

Index

 

WP 6 and WP 7.3

Articles

Martínez-Jiménez C. P., Jover R., Donato M. T., Castell J.V. and Gómez-Lechón M. J.. (2007). CYP3A4 regulation and expression in hepatocytes Curr Drug Metab. 8:185-195

Gómez-Lechón M.J., Lahoz A. and Donato M.T. (2007). In vitro screening of drug metabolism in drug development. SCREENING Trends in Drug Discovery 8:32-33

Gómez-Lechón M.J. , Donato M. T., Martínez-Romero A., Jiménez N., Castell J. V. and O´Connor E.. (2007). A human hepatocellular in vitro model to investigate steatosis. Chemico-Biol Interact 165:106-116

Donato M. T., Jiménez N., Serralta A., Mir J., Castell J. V and Gómez-Lechón M. J.. (2007). Effects of steatosis on drug-metabolizing capability of primary human hepatocytes. Toxicol In vitro 21: 271-276

Gómez-Lechón M. J. , Castell J. V. and Donato M. T.. (2007). Hepatocytes the choice to investigate drug metabolism and toxicity in man: In vitro variability as a reflection of in vivo. Chemico-Biol Interact 168:30-50

Zdarilová A., Cvek B., Vrba J., Gómez-Lechón M.J. and Ulrichová J. (2008). Cytotoxicity of model compounds on cell lines (3T3, HepG2) and rat hepatocytes. Biomedical Papers, Vol. 1.

Lahoz A., Donato M. T., Montero S., Castell J. V. and Gómez-Lechón M. J. (2008). A new in vitro approach for the simultaneous determination of phase I and phase II enzymatic activities of human hepatocyte preparations. Rapid Commun. Mass Spectrom. 22: 240–244.

Gómez-Lechón M. J., Lahoz A., Castell J. V. and Donato M. T. (2008). Cell lines: A tool for in vitro drug metabolism studies Curr Drug Metab. 9:1-11.

Gui C., Miao Y., Thompson L., Wahlgren B., Mock M., Stieger B. and Hagenbuch B. (2008). Effect of pregnane X receptor ligands on transport mediated by human OATP1B1 and OATP1B3. European Journal of Pharmacology 584, 57–65.

Lahoz A., Donato M. T., Castell J. V and Gómez-Lechón M. J. (2008). Strategies to in vitro assessment of major human CYP enzyme activities by using liquid chromatography tandem mass spectrometry. Curr Drug Metab. 9:12-19.

Rohacova J., Marin M.L., Martínez-Romero A., O'Connor J.E., Gomez-Lechon M.J., Donato M.T., Castell J.V., Miranda M.A. (2008). Photophysical characterization and flow cytometry applications of cholylamidofluorescein, a fluorescent bile acid scaffold. Photochem Photobiol Sci. 7:860-866.

Gómez-Lechón M. J., Castell J. V. and Donato M. T. (2008). An update on metabolism studies using human hepatocytes in primary culture. Expert Opin Drug Metab Toxicol 4: 837-854.

Lahoz A., Donato M.T., Picazo L., Castell J.V. and Gómez-Lechón M.J. (2008). Assessment of cytochrome P450 induction in human hepatocytes using the cocktail strategy plus liquid chromatography tandem mass spectrometry. Drug Metab Letters 2:205-209.

Lahoz A., Donato M.T., Montero S., Castell J.V. and Gómez-Lechón M.J. (2008). In vitro cocktail assay for the simultaneous determination of phase I and phase II enzymatic activities of human hepatocyte preparations. Rapid Communications in Mass Spectrometry 22: 240–244

Pascual M., Gómez-Lechón M.J., Castell J.V. and Jover R. (2008). ATF5, a highly abundant liver-specific transcription factor, cooperates with CAR in the transactivation of CYP2B6: Implications in hepatic stress response. Drug Metab Disp 36:1063-1072.

Rohacova J., Marín M.L., Martinez-Romero A., Diaz L., O'Connor J.E., Gomez-Lechon M.J., Donato M.T., Castell J.V., Miranda M.A. (2009). Fluorescent Benzofurazan-Cholic Acid Conjugates for in vitro Assessment of Bile Acid Uptake and Its Modulation by Drugs. ChemMedChem. 4:466-472

Rohacova J., Marín M.L., Martinez-Romero A., O'Connor J.E., Gomez-Lechon M.J., Donato M.T., Castell J.V., Miranda M.A.. (2009). Synthesis of new, UV-photoactive dansyl derivatives for flow cytometric studies on bile acid uptake Org. Biomol. Chem .7:4973-80.

Gómez-Lechón M. J., Lahoz A., Gombau L., Castell J.V. and Donato M. T. (2010). In vitro evaluation of potential hepatotoxicity induced by new drugs. Curr Pharm Design 16:1963-77

de Waart D.R., Häusler S., Vlaming M.L., Kunne C., Hänggi E., Gruss H.J., Oude Elferink R.P., Stieger B. (2010). Hepatic transport mechanisms of cholyl-L-lysyl-fluorescein. J Pharmacol Exp Ther. 334(1):78-86.

Stieger B. (2010). Role of the bile salt export pump, BSEP, in acquired forms of cholestasis. Drug Metab Rev. 42(3):437-45.

Submitted manuscripts

Donato M.T., Hallifax D., Picazo L., Castell J.V., Houston J.B., Gomez-Lechon M.J. and Lahoz A.. (2009). Metabolite formation kinetics and intrinsic clearance of phenacetin, tolbutamide, alprazolam and midazolam in adenoviral P450 transfected HepG2 cells and comparison with hepatocytes and in vivo. Drug Metab Disp (in press)

Donato M.T., Castell J.V. and Gómez-Lechón M.J.. In vitro P450 inhibition/induction in early drug Development. M Expert Opinion on Drug Metabolism and Toxicology (in press)

Submitted manuscripts

Baranczewski P., Long A., Lundgren B., Svensson R. and Garberg P.  Biotransformation of the piperazine ring and an evaluation of the expert system Meteor. 9th ISSX meeting, Manchester, UK, June 4-7, 2006. Drug Metabolism Reviews, volume 38, supplement 1, 147, 2006.

Conference contributions

Baranczewski P., Long A., Lundgren B., Svensson R. and Garberg P. Biotransformation of the piperazine ring and an evaluation of the expert system Meteor. 9th ISSX meeting, Manchester, UK, June 4-7, 2006. Drug Metabolism Reviews, volume 38, supplement 1, 147, 2006.

Castell J. Can dendritic cells metabolise (bioactivate) drugs? Clues to investigate in vitro the onset of an hypersensitivity reaction. Oral presentation at the 14th International Workshop on In Vitro Toxicology, Ostende, Belgium, 2-5 October, 2006.

Perez-Cataldo G., Castell M., Rodriguez-Pena R., Lopez S., Mayorga L., Blanca M. and Castell J.V. Can dendritic cells metabolise (bioactivate) drugs?: clues to investigate in vitro the onset of an hypersensitivity reaction. 14th International Workshop on In Vitro Toxicology, Ostende, Belgium, 2-5 October 2006. Oral presentation.

Ulrichová J., Cvek B., Zdarilová A., Vrba J. and Gómez-Lechón M.J. In vitro models in the prediction of metabolism and target organ specifity. III Meeting of the A-Cute-Tox Consortium,

Ostende, October 2006. Poster. Zdarilová A., Cvek B., Gómez-Lechón M.J. and Ulrichová J. Cytotoxicity of SAnguinarine and chelerythrine on different cell models. 24th SSCT Workshop on in vitro toxicology, September 7-9th, Grinda, Stockholm 2006. Poster.

Cvek B., Vrba J., Gómez-Lechón M.J. and Ulrichová J. In vitro models in the prediction of hepatoxicity in man. 46th ETCS International Meeting/3rd International Joint Meeting AICC-Celltox on "In Vitro Cytotoxicity Mechanisms" Verona, March 2006. Poster.

Cvek B., Zdarilová A., Vrba J., Gómez-Lechón M.J. and Ulrichová J. Cytotoxicity of model compounds on cell lines (3T3, HepG2) and rat hepatocytes. 25th SSCT Workshop on in vitro toxicology, 19-22 September Salzau, Germany 2007.

Pinto S., Diaz L., Herrera G., Gomez-Lechón M.J., Donato T., Castell J.V., Callaghan R.C.and O’Connor J.E. Application of high-content analysis to explore functional endpoints of cytotoxicity in primary cultures of hepatocytes. Workshop "High Content Analysis",Madrid, March 2007.

Index

 

WP 7.1: Role target organ toxicity (brain)

Articles

Forsby A. and Blauuboer B. (2007) Integration of In Vitro Neurotoxicity Data with Biokinetic Modeling for the Estimation of In Vivo Neurotoxicity. Human and experimental toxicology, 26; 333-338.

Yeste-Velasco M., Alvira D., Sureda F.X., Rimbau V., ForsbyA., Pallàs M., Camins A., Folch J. (2008). cDNA low density array analysis of colchicine neurotoxicity in rat cerebellar granular neurons. Neurotoxicology, 29(2):309-17.

Suñol C., Babot Z., Fonfría E., Galofré M., García D., Herrera N., Iraola S., Vendrell I. (2008). Studies with neuronal cells: from basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity‖ by Toxicology In Vitro. 22 (5), 1350-5.

Bal-Price A.K., Suñol C., Weiss D.G., van Vliet E., Remco H.S. (2008). Application of in vitro neurotoxicity testing for regulatory purposes: Symposium III Summary and Research Needs. Neurotoxicology. 29(3), 520-31.

Forsby A., Bal-Price A., Camins A., Coecke S., Fabre N., Gustafsson H., Honegger P., Kinsner-Ovaskainen A., Pallas M., Rimbau V., Rodríguez-Farré E., Suñol C., Vericat J.A. and Zurich M.G. (2009). Neuronal in vitro models for the estimation of acute systemic toxicity. Toxicology In Vitro , 23, 1564-9.

Folch J., Alvira D., López-Querol M., Tajes M., Sureda F.X., Forsby A., Rimbau V., Camins A., Pallas M. (2009). Evaluation of transcriptional activity of caspase 3 gene as a marker of acute neurotoxicity in rat cerebellar granular cells. Toxicology In Vitro, 24, 465-71.

Folch J., Yeste-Velasco M., Alvira D., de la Torre A.V., Bordas M., López M., Sureda F.X., Rimbau V., Camins A., Pallàs M. (2009). Evaluation of pathways involved in pentachlorophenol-induced apoptosis in rat neurons. Neurotoxicology 30(3):451-8.

Aschner M., Levin E.D., Suñol C., Olopade J.O., Helmcke K.J., Avila D.S., Sledge D., Ali R.H., Upchurch L., Donerly S., Linney E., Forsby A., Ponnoru P., Connor J.R. (2010). Gene-Environment Interactions: Neurodegeneration in Non-Mammals and Mammals. doi:10.1016/j.neuro.2010.03.008.

Gustafsson H., Runesson J., Lundqvist J., Lindegren H., and Forsby A. (2010). Neurofunctional endpoints assessed in human neuroblastoma SH-SY5Y cells for estimation of acute systemic toxicity. Toxicology and Applied Pharmacology, 245(2): 191-202.

Galofré M., Babot Z., García D.A., Iraola S., Rodrìguez-Farré E., Forsby A., Suñol C. (2010). GABAA receptor and cell membrane potential as functional endpoints in cultured neurons to evaluate chemicals for human acute toxicity. Neurotoxicology and Teratology 32, 52-61.

Manuscripts

Honegger P., Tavel D., Zurich M.-G. Aggregating brain cell cultures as a model for acute neurotoxicity testing. Submitted to NeuroToxicology.

Conference contributions

Forsby A. An in vitro test strategy for prediction of acute neurotoxicity. 23rd SSCT workshop in collaboration with the Estonian Toxicology Society, Toila, Estonia 2005. Oral presentation. Forsby A. Integration of In Vitro Neurotoxicity Data with Biokinetic Modeling for the Estimation of In Vivo Neurotoxicity. 10th workshop of the International Neurotoxicology Association, Poorvo, Finland 2005. Oral presentation.

Gustafsson H., Runesson J. and Forsby A. Acetylcholine esterase activity as an endpoint for prediction of acute human neurotoxicity. 23rd SSCT workshop in collaboration with the Estonian

Toxicology Society, Toila, Estonia 2005. Oral presentation.

Gustafsson H., Runesson J. and Forsby A. Prediction of human acute neurotoxicity – development of a test strategy in vitro. 23rd SSCT workshop in collaboration with the Estonian Toxicology Society, Toila, Estonia 2005. Oral presentation.

Forsby A., Gustafsson H., Honegger P., and Sunol C. Receptor-mediated acute systemic toxicity studied in neuronal cell models. Abstract and platform presentation at the 24rd SSCT workshop in Stockholm 7-9 September 2006.

Gustafsson H. and Forsby A. Cell membrane potential – a promising endpoint for the prediction of acute human neurotoxicity. Abstract and platform presentation at the 24rd SSCT workshop in Stockholm 7-9 September 2006.

Yeste M., Alvira D., Sureda F.X., Folch J., Crespo-Biel N., Rimbau V., Camins A. and Pallàs M. Evaluation of neurotoxicity in cerebellar granule cells. An In Vitro approach to In Vivo Toxicity. Simposi de la Societat Catalana de Neurobiologia held in Barcelona, December 2006. Poster.

Suñol C., García D.A. Iraola S. and Babot Z. Assessment of neurochemical end-points in primary neuronal cultures to predict acute toxicity of neurotoxic compounds. VI Symposium of Experimental Neurobiology. Catalan Society of Biology. Barcelona, Spain. 22 – 23 November 2006. Poster.

Suñol C. In vitro neural models to evaluate human acute neurotoxicity. The European project ACuteTox. 11th Meeting of the International Neurotoxicology Association, Asilomar Conference Center, Pacific Grove, California, USA, 10 – 15 June 2007. Oral presentation.

Rodríguez-Farré E., Pertusa M., García-Matas S., Suñol C., Sanfeliu C. and Cristòfol R. Astrocytes aged in vitro display an impaired neuroprotective capacity in cortical neurons: a tool in screening for neurotoxicity. 11th Meeting of the International Neurotoxicology Association, Asilomar Conference Center, Pacific Grove, California, USA, 10 – 15 June 2007. Poster.

Gustafsson H., Runesson J., Lindegren H., Axelsson V. and Forsby A. The use of the human neuroblastoma SH-SY5Y cell line for estimation of acute systemic toxicity.. 25th SSCT Workshop on in vitro toxicology, 19-22 September Salzau, Germany 2007. Oral presentation.

Suñol C. Studies with neuronal cells: from basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity. 25th SSCT Workshop on in vitro toxicology, 19-22 September Salzau, Germany 2007. Oral presentation.

Pallàs M. Drug-induced neurotoxicity regulates apoptosis, oxidative stress and cell cycle control gene expression. XXIX Meeting of the Spanish Society for Pharmacology, Alcalá de Henares, Spain, September 2007. Poster.

Galofré M., García D., Babot Z., Iraola S., Collazo O., Herrera N. and Suñol C. Neuronal endpoints in primary neuronal cultures to evaluate and predict human acute neurotoxicity. XII meeting of the Spanish Society for Neuroscience, Valencia, Spain, 5-7 September 2007. Poster.

Babot Z., Galofré M., García D., Herrera N., Iraola S., Sabater D. and Suñol C. The GABAA and NMDA-glutamate receptors as targets of toxicity. XXVIII Meeting of the Spanish Group on Neurotransmission (GEN), Aranjuez, Spain, 12-14 December 2007. Oral presentation.

Forsby A., Camins A., Coecke S., Fabre, N. , Gustafsson H., Honegger P., Kinsner-Ovaskainen A., Pallas M., Price A., Rimbau V., Rodríguez-Farré E., Suñol C., Vericat J.A.and Zurich, M.G. The 15th International Congress on In Vitro Toxicology, 25 to 28 Sept 2008, Stockholm, Sweden (ESTIV2008). Abstract and invited speaker. Neuronal in vitro models for the estimation of acute systemic toxicity.

Lundqvist J., Runesson J., Gustafsson H. and Forsby A. The influence of exposure time on the inhibitory effect of the acetylcholine esterase activity in human neuroblastoma SH-SY5Y cells. The 15th International Congress on In Vitro Toxicology, 25 to 28 Sept 2008, Stockholm, Sweden. Poster presentation.

Galofré M., Babot Z., García D.A., Iraola S., Herrera N. and Suñol C. GABAA receptor and cell membrane potential as functional endpoints in cultured neurons to evaluate chemicals for human acute toxicity. 45th Congress of the European Societies of Toxicology (EUROTOX 2008). October 2008, Rodes, Greece.

Galofré M., Babot Z., García D.A., Iraola S., Herrera N., Suñol C. GABA-A receptor and cell membrane potential as functional endpoints in cultured neurons to evaluate chemicals for human acute toxicity. VII Symposium of Experimental Neurobiology. Catalan Society of Biology. Barcelona, Spain. November 2008.

Folch J., Rimbau V., Alvira D., Sureda F.X., Camins A. and Pallàs M. Caspase-3 gene: sensor of acute neurotoxicity. The XXX Congreso de la Sociedad Española de Farmacologia, september 2008, Bilbao. Spain. Poster presentation

Suñol C. and Forsby A. Combination of cell based assays determining GABAA receptor activity and Cell Membrane Potential for in vitro testing of neurotoxicity. Forinvitox event May 12-14. Stockholm, 2009. Oral presentation.

Suñol C. and Forsby A. Way forward for using in vitro neurotoxicity models in testing strategy: update of FP6 integrated Project ―ACuteTox‖. 12th Meeting of the International Neurotoxicology Association. Ma’ale Hachamisha, Israel, 7-12 June 2009. Oral presentation

Suñol C. Cell-based in vitro neurotoxicity assays to predict human toxicity. XII International Congress of Toxicology. 19 – 23 July, Barcelona, Spain

Lectures

Advanced course in Cellular and Genetic Toxicology at Stockholm university:
a. Applications of toxicological methods in cell culture
b. Mechanisms of neurotoxicology

Suñol C. In vitro assays to predict neurotoxicity. Master in Mental Health: Research in Psychiatry, Neurotoxicology and Psychopharmacology. University Rovira I Virgili. Spain.

Others

Hörnblad L. and Runesson J. Establishing a method to determine neurospecific toxicity by measuring the AchE-activity in human neuroblastoma SH-SY5Y cell. Advanced course project, Neurochemistry and neurotoxicology, Stockholm university, 2005. Student report.

Runesson J. A toxicity study measuring the AChE Activity in human neuroblastoma SH-SY5Y cells. Project Report, Stockholm university, 2005. Student report.

Mukuru A. and Stjärnmark-Hirschberg L. Determining noradrenalin uptake in human neuroblastoma cells (SH-SY5Y) after exposure to amphetamine and caffeine. Advanced course project, Stockholm university, 2006. Student report.

Index

 

WP 7.2: Role target organ toxicity (kidney)

Articles

McMorrow T., Gaffney M.M., Slattery C., Campbell E. and Ryan M.P. (2005). Cyclosporine A - induced epithelial to mesenchymal transdifferentiation (EMT) in renal tubular epithelial cells. Nephrol. Dial. Trans. 20(10):2215-25.

Slattery C., Campbell E., McMorrow T. and Ryan M.P. (2005). Cyclosporine A - induced renal fibrosis: a role for epithelial to mesenchymal transdifferentiation. Am. J. Path. 167(2): 295-407.

Campbell E., Slattery C., Tuite N., Nolan S., Leonard M., Ryan M.P. and McMorrow T. RNA Interference: a Novel Alternative Approach in Nephrotoxicity Studies. 5th World Congress on Alternatives & Animal Use in the Life Sciences, Berlin, August 21-25, 2005. ALTEX 23, Special issue 2006.

Slattery C., Campbell E., McMorrow T. and Ryan M.P. (2006) Over-expression of E2A proteins induces epithelial mesenchymal transition in human renal proximal tubular epithelial cells suggesting a potential role in renal fibrosis. FEBS Letters 580:4021-4030.

Low C.A., Ryan G., McEwan G., Duff T., Ryan M.P., Hawksworth G.M. (2006). Comparative analysis of indices of in vitro toxicity in a renal cell line, Toxicology, 226, 57-58

Alderson C.A., Ryan G., McEwan G., Ryan M.P., Hawksworth G.M. (2007). Transepithelial resistance and transporters in human and porcine renal cell lines. Toxicology, 240, 162-163

Young L.C., Hawksworth G.M., Alderson C.A. (2008). Diquat alters the localisation of E-cadherin, B-catenin, occludin and ZO-1 in LLCPK1 cells Toxicology, 253, 21

Slattery C., Ryan M.P. and McMorrow T. (2008). E2A proteins - regulators of cell phenotype in normal physiology and disease. International Journal of Biochemistry & Cell Biology 40(8):1431-6.

Martin-Martin M., Ryan G., McMorrow T. and Ryan M.P. (2010). Sirolimus and CsA alter barrier function in renal proximal tubular cells through stimulation of ERK1/2 signaling and claudin-1 expression Am J Physiol Renal Physiol 298 F672-F682

Manuscripts

McMorrow T., Campbell E., Slattery C. and Ryan M.P. Molecular mechanisms modulating epithelial-mesenchymal transition (EMT) in human renal epithelial cells. Cell Physiology: Human epithelial neoplastic cell plasticity: Implications for tumor progression and metastasis. Editor: Paul

J. Higgins, PhD (in press). Manuscript.

Slattery C., O’Connell S., Ryan M.P. and McMorrow T. The Role of PKC- in Cyclosporine A-Induced Tubulointerstitial Fibrosis. International Journal of Biochemistry & Cell Biology. (in press). Manuscript.

Conference contributions

Low C.A., Ryan G., McEwan G., Ryan M.P. and Hawksworth G.M. Optimisation of an in vitro test strategy for predicting acute Nephrotoxicity. 14th International Workshop on In Vitro Toxicology, Ostende, Belgium, 2-5 October 2006.

Martín N., Ryan G., Anderson C., Hawksworth G. and Ryan M.P. Optimisation and pre-validation of an in vitro test strategy for predicting human acute toxicity. 6th World Congress on Alternatives & Animal Use in the Life Sciences, Tokyo, Japan, 20th August 2007. Poster.

Martín N., Ryan G. and Ryan M.P. Optimisation and pre-validation of an in vitro test strategy for predicting human acute toxicity. UCD Conway Festival of Research, Dublin, Ireland, 20th September 2007. Poster.

Martín N. The effect of Cyclosporine A and Rapamycin on the integrity of the cellular tight-junctional complex in renal proximal tubular cells Conway Institute Research Festival, Dublin, Ireland, 5th September 2007. Oral presentation.

Index

 

WP 9: Pre-validation of test strategy

Conference contribution

Kopp-Schneider A. Statistical analysis of the ACuteTox data: challenges and statistical approaches. VII World Congress on Alternatives & Animal Use in the Life Sciences. Roma (Italy), August 30- September 3, 2009. Oral presentation. ALTEX, 26 (Spec. Issue) 1-376 (2009) pp 269-270.

Index